Journal: International Journal of Molecular Sciences

Article Title: Development of a Vaccine Candidate Based on Surface-Displayed Particles of Mycobacterium tuberculosis from the MTB39A Protein

doi: 10.3390/ijms26020797

Figure Lengend Snippet: T cell proliferation and safety evaluation of mice immunized with nanoparticle vaccines. ( A ) Flow cytometer gate strategy. ( B ) Percentage of CD4 + /CD8 + T cells in splenic lymphocytes of immunized mice, ( C ) CD3 + IL-4 + T cells, ( D ) CD4 + IL-4 + , ( E ) CD3 + IFN-γ + , ( F ) CD4 + IFN-γ + , and ( G ) CD8 + IFN-γ + . ( H ) Haematoxylin–eosin (HE) staining of tissue sections of major organs (10×, n = 3). The data are shown as the means ± SDs and analyzed by two-way ANOVA using the Bonferroni test. ns, not significant; * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: Splenocytes were isolated 42 days after immunization, inoculated into 6-well plates at 1 × 10 6 cells per well, and stimulated in vitro with 5 μg/mL of rvAc-71CA and rvAc-MTB39A for 12 h. The cells were centrifuged at 1000 rpm for 5min, collected, and then used for surface staining (Proteintech, Wuhan, China): CoraLite ® Plus 405 Anti-Mouse CD4 (GK1.5, Proteintech, Wuhan, China)), PE Anti-Mouse CD3 (17A2, Proteintech, Wuhan, China)), and CoraLite ® Plus 705 Anti-Mouse CD8a (53-6.7, Proteintech, Wuhan, China)).

Techniques: Vaccines, Flow Cytometry, Staining